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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in many tumors, which ends up in chromosome instability, and therefore may add to tumorigenesis. This protein was uncovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial purpose in promoting mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been described. Two additional splice variants were reported but detailed nucleotide sequence information is not nonetheless obtainable.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide normally consists of the epitope regarded by the antibody.
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This guideline outlines further more achievable optimization of cellular and immunoassay parameters to be sure the best possible benefits are...
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This tutorial outlines further more achievable optimization of mobile and immunoassay parameters to make sure the best possible effects are...
Antibodies certain to the blocking peptide now not bind to your epitope on the goal protein. By comparing the staining through the blocked antibody as opposed to the antibody alone, you can see which staining is specific.
Concentrate on Info Cyclin E belongs into the hugely conserved cyclin spouse and children, more info whose users are characterised by a remarkable periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation designs which lead on the temporal coordination of each and every mitotic event. Cyclin E kinds a complex with and functions for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary which is degraded as cells progress by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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